BULKGLP RESEARCH LIBRARY
BPC-157: molecular identity and analytical reference
Published
BPC-157 identity and reference structure
BPC-157 is a synthetic, linear pentadecapeptide: a chain of fifteen amino acid residues. Its reference sequence is GEPPPGKPADDAGLV, written from the amino terminus to the carboxyl terminus. The expanded notation H-Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val-OH specifies unmodified terminal groups. Sequence, terminal chemistry and chemical form belong in an identity specification; the name BPC-157 alone is a shorter label for that specification.
The figures below describe the parent peptide recorded in PubChem. They exclude acetate, trifluoroacetate, residual water and other material that may accompany a supplied powder. A reference molecular weight is therefore a comparison value for the defined molecule, rather than a measurement of the contents or purity of a particular vial.
| Reference compound | BPC-157; linear 15-residue peptide |
|---|---|
| Parent CAS registry number | 137525-51-0 |
| Parent molecular formula | C62H98N16O22 |
| Parent molecular weight | 1419.5 g/mol (PubChem average molecular weight) |
| Reference sequence | H-GEPPPGKPADDAGLV-OH |
| Supplied counterion and hydration | Not established by these parent-molecule identifiers; consult the lot specification |
Research models and what they measure
Published BPC-157 work includes experiments on cultured rat tendon fibroblasts. Chang and colleagues assessed cell migration, actin organization and phosphorylation of focal adhesion kinase and paxillin. Those endpoints address specific cellular responses within the reported model. They do not establish that another preparation reproduces the experiment, and they are not evidence of a guaranteed outcome from the material offered here.
Analytical researchers have also investigated BPC-157 metabolism using isotope labeling and ultra-high-performance liquid chromatography with high-resolution mass spectrometry. Metabolite identification is distinct from a release assay for an unopened vial. When comparing papers, retain the sample matrix, analytical platform and experimental conditions alongside the reported result.
Reading identity, purity and content together
A mass-spectrometry result should state the measured ion, its charge state and the theoretical mass used for comparison. A multiply charged ion is reported as mass-to-charge ratio, so its displayed number need not equal the neutral molecular weight. A matching intact mass supports identity but does not independently establish every residue position or stereochemical detail.
HPLC purity describes the detected peaks under the stated separation and integration method. Quantitative peptide content answers a different question: how much specified peptide was measured in the submitted sample. Evaluate both findings with their methods and sample identifiers. A high chromatographic percentage cannot, by itself, convert a nominal vial label into a measured amount.
For a reproducible comparison, retain the method version and the reference material used by the laboratory. Two reports with different separation conditions or mass conventions may not be directly comparable even when both use the BPC-157 name. Resolve those differences before interpreting a discrepancy as a change in material quality.
Format, handling records and lot selection
The catalog lists a nominal 10 mg research vial. Nominal amount, parent molecular weight and measured sample content are separate fields. Match the received product and lot to its report before using any reported result in a laboratory record. Do not transfer a result from a different size, formulation or historical lot.
Record the supplied chemical form, receipt date, seal condition and documented storage or retest requirements. Experimental sample preparation should follow a method suitable for the intended analysis. This reference does not assign a universal solution stability, storage temperature or expiry period; those require evidence for the actual material and conditions.
Frequently asked questions
Does the CAS number prove the identity of a received vial?
No. A registry identifier defines the intended reference substance. Evidence for the received material must come from its traceable sample documentation and suitable analytical results.
Are the parent peptide and an acetate preparation identical specifications?
No. They share a peptide moiety, but a salt specification must also address its counterion and amount. The parent formula and mass shown above do not quantify acetate or water.
Primary sources and chemical records
- PubChem: BPC-157, CID 9941957
- Chang et al.: BPC-157 in tendon fibroblast experiments (2011)
- Isotope labeling and UHPLC-HRMS characterization of BPC-157 metabolism (2023)
- USP researchers: reference standards and quality characterization of synthetic peptides (2023)
Prepared by BulkGLP. Editorial review: 2026-11-04.